A factors that may affect communication Essay

Often when we communicate there is always an overall power within that communication. The speaker may create the status of ring leader. This power throughout communication may be seen as a strength, as they are leading the conversation, however this power may be perceived negatively. The recipient in the conversation may feel small and belittled as there is no chance for them to talk, this may therefore cause communication to be less effective. In health and social care both the care provider and patients have power. The patient would have power over final decisions that need to be made concerning their care, however the care provider would have power over the level of care given. This balance of power should always be shared positively. Power throughout communication plays an important role. The care giver should always take the lead. It is important for the patient to have their say on any decisions that may be made, however if the care provider feels they are making the wrong deci sion they should use their all their power of medical intelligence to persuade the patient to make the right decision. Abuse of power may be seen in the workplace. As an example a care worker may be the subject of abuse) by senior management. The senior management may think that the care worker won’t tell anyone due to his or her higher status. This would be an abuse of power on their behalf as they are using their higher level of management to intimidate. This care workers communication throughout the job may also suffer due to their being in a vulnerable state. A strategy that may help any use and abuse of power being stopped may be to promote individual rights within the workplace. This could help to ensure everyone is clear of what help they could have. Putting staff in touch with trade unions may also help. In relation to the above-mentioned case study, an abused care worker might apply the strategy  of speaking to someone in higher management. If the care worker did not feel comfortable doing this, another idea might be to report it to a complaint company or the police. Reporting to higher management may be seen as a positive or a negative. The positive to this strategy is that fast action could take place to ensure the problem is resolved. However the negative could be that the situation isn’t kept private as each different manger might have to discuss it, this may in turn cause the care worker to feel embarrassed and unsafe. In conclusion I feel the use and abuse of power is a key consideration for the care worker and one that will certainly need to be applied strategically. The use of various strategies to meet various situations will create an image of the personality of the care worker in the eyes of their patient. The final factor which effects effective communication would be the use of alcohol and drugs. When having to care for persons under the influence of these substances the lack communication will become a great struggle and may cause great difficulties. A case study example could be in an A and E unit. If the carer is dealing with a person under the influence they would have to be very patient. The person receiving the care may be angry, the best way to defuse this anger is to make sure the person has enough space, if they feel overcrowded this may make them more angry as they are unaware of what is going on. As a carer you would ensure yourself to talk slowly, you may also find that you have to often repeat yourself. While communicating with someone who has drank or taken substances they may either be quiet and be struggling to talk or they may say things that don’t make any sense. As a carer you would try to make them understand where they are and what is going on. Reassuring may gain trust as they know you are only trying to help. If a carer had to undertake a procedure while the patient was under the influence communication would be key. Making sure the person understands what is happening and why is very important. If the person cooperates this will make the procedure a lot easier however there could be a possibility that the person may decide half way through the procedure that they no longer want it or as the drugs or alcohol are wearing off they may feel more confused. Confusion could cause the patient to become angry against the person who is caring for them. Their only defence mechanism they  may feel they have to defend their- selves may be to act physically against the carer. To help ensure this does not happen communication throughout the whole process is vital. However if the patient still reacts angrily having another carer there should ensure more safety. By making the patient aware of their surroundings could bring both negatives and positives. The positive side to this is the patient is aware of where they are, this may help by them being more comparative in any procedures that may need to take place. However this action could also be looked at negatively by telling the patient about their surroundings might make them not want to be there this may cause their cooperation to be poor. Receiving effective communication with a persons under the influence of drink and drugs can be proven difficult however talking slowly and using appropriate strategies should ensure the communication is as effective as possible.

Thinking Errors Found and Fixed

The Franklin Reality Model is a modeling technique that works to restructure thinging errors by way of cognitive thinking. Process is a step by step process where as you have a decision to make and you are given a few decisions, you make the one that you think will work, for whatever reason. Step two of this process is deciphering what effects come about from that decision, effectively you will begin to see a ripple effect based upon your choice.This ripple effect can be positive or negative, once again depending upon your choice but the technique gets one to thinking about the simple effects of what seems to be a simple solution until they learn to start thinking ahead about their actions before they act. To live, to love and be loved, to feel important, and variety are the basic components of this model. There are also seven natural laws within this model and they are 1. If the results of your behavior do not meet your needs, there is an incorrect principle on your belief window.2) Results take time to measure. 3) Growth is the process of changing principles on your belief window. 4) If your self-worth is dependent on anything external, you are in big trouble. 5) Addictive behavior is the result of deep and unmet needs (of the four human needs mentioned above). 6) The mind will naturally seek harmony when presented with two opposing principles. 7) When the results of your behavior do meet your needs you experience inner peace. (Franklin Reality Model)In this model we place principles upon our beliefs. For example Saving money is a waste because I’m going to spend it anyway is a belief on a principle. With this thought then you can be assured that you won’t save any money and there will come a time when a repair on a vehicle is needed, or you lose a job and have no money to pay your bills; when this happens then what? This model has been effectively used and has effectively altered people’s way of thinking.Those with behaviorial issues to those with addiction issues have benefitted from this and the developers of this program use it with a high rate of recidivism within the prison systems. This gets to the root of an issue, of a bad thought pattern, how can one ignore this and at least not try. It is an eye opener exercise for everyone. References FRANKLIN REALITY MODEL. AS COGNITIVE RESTRUCTURING. 1986. http://www. gwcinc. com/C_Reddick/FranklinRealityModelAsCognitiveRestructuring. htm

Motivation and Leadership Effect on Job Performance Essay

Introduction Leadership and motivation traditionally play a very important role in the functioning of organizations. Nowadays, the role of leadership and motivation increases even more significantly because of the growing competition and the increasing significance of human resources for the progress of organizations. In fact, it should be pointed out that leadership and motivation are closely entangled concepts which constitute an important part of the success of an organization. It is obvious that the effective leadership and motivation can contribute to the progress of an organization, while poor leadership and the lack of motivation can weaken the normal functioning of an organization and lead to the crisis within an organization that will naturally affects its performance. In this respect, it is important to underline the fact that leadership and motivation affect considerably the organizational behavior and culture and they define the formation of the effective relationships between the personnel of an organization. In such a situation, it is necessary to fully reveal the significance of leadership and motivation in the current business environment in order to assess adequately perspective of the improvement of the effectiveness of leadership and motivation within an organization. At the same time, the analysis of theoretical framework only, it appears that insufficient for the understanding of the significance of leadership and motivation for the performance of an organization. Consequently, it is also necessary to pay a particular attention to the practical aspects of leadership and motivation  that means that it is necessary to analyze some practical examples that could reveal the significance of leadership and motivation for the performance of a n organization Review of Literature Motivation and Job Performance * The level of performance of employees relies not only on their actual skills but also on the level of motivation each person exhibits (Burney et al., 2007). * Motivation is an inner drive or an external inducement to behave in some particular way, typically a way that will lead to rewards (Dessler, 1978). * Over-achieving, talented employees are the driving force of all firms so it is essential that organizations try hard to motivate and hold on to the best employees (Harrington, 2003). * The quality of human resource management is a critical influence on the performance of the firm. Concern for strategic integration, commitment flexibility and quality, has called for attention for employee’s motivation and retention. Financial motivation has become the most concern in today’s organization, and tying to apply Maslow’s basic needs, non-financial aspect only comes in when financial motivation has failed. * According to Greenberg and Baron (2003, 2000) definition of motivation could be divided into three main parts. The first part looks at arousal that deals with the drive, or energy behind individual (s) action. People turn to be guided by their interest in making a good impression on others, doing interesting work and being successful in what they do. The second part referring to the choice people make and the direction their behavior takes. The last part deals with maintaining behavior clearly defining how long people have to persist at attempting to meet their goals. * Motivation can be intrinsic and extrinsic. Extrinsic motivation concerns behavior influenced by obtaining external rewards (Hitt, Esser, & Marriott, 1992). Praise or positive feedback, money, and the absence of punishment are examples of extrinsic or external rewards (Deci, 1980). Intrinsic motivation is the motivation to do something simply for the pleasure of performing that particular activity (Hagedoorn and Van Yperen, 2003). Examples of intrinsic factors are interesting work, recognition, growth, and achievement. *  Several studies have found there to be a positive relationship between intrinsic motivation and job pe rformance as well as intrinsic motivation and job satisfaction (Linz, 2003). This is significant to firms in today’s highly competitive business environment in that intrinsically motivated employees will perform better and, therefore, be more productive, and also because satisfied employees will remain loyal to their organization and feel no pressure or need to move to a different firm. * Deci and Ryan (2000) conducted and replicated an experiment that showed the negative impact of monetary rewards on intrinsic motivation and performance. A group of college students were asked to work on an interesting puzzle. Some were paid and some were not paid for the work. The students that were not being paid worked longer on the puzzle and found it more interesting than the students being paid. When the study was brought into a workplace setting, employees felt that their behavior was being controlled in a dehumanizing and alienating manner by the rewards. It was discovered that rewards would seriously decrease an employee’s motivation to ever perform the task being rewarded, or one similar to it, any time in the future. Another observation of the study was that employees would expect a reward every time the task was to be completed if the reward was offered at one time. Employees would require the reward in order to perform the job and would probably expect the reward to increase in amount. If the rewards were not increased or if they were taken away they actually served as negative reinforcement. * Deci and Ryan (2001), in collaboration with two of their colleagues, conducted a study to examine the effects of performance-contingent rewards on an employee’s intrinsic motivation. * (Cameron, Deci, Koestner, and Ryan, 2001). These types of rewards are very controlling since these rewards are directly associated with an employee’s performance of some task. In that respect, performance-contingent rewards undermine intrinsic motivation; however, if the reward given to the employee conveys that the employee has performed on a truly outstanding level, the reward would serve to solidify that employee’s sense of competence and decrease the negative effect on that employee’s intrinsic motivation. Also significant in the study was the importance of the interpersonal atmosphere within which the performance-contingent rewards were distributed. * When Cameron et al. (2001) compared the administering of rewards in a controlling climate and in  a non-controlling climate, they discovered that the performance-contingent rewards given in the more controlling interpersonal climate undermined intrinsic motivation. To prevent the decrease in employees’ intrinsic motivation, the interpersonal climate when distributing performance-contingent rewards should be more supportive. * Vroom (1964) proposes that people are motivated by how much they want something and how likely they think they are to get it he suggest that motivation leads to efforts and the efforts combined with employees ability together with environment factors which interplay’s resulting to performance. This performance interns lead to various outcomes, each of which has an associated value called Valence. * Adams (1965) on his part suggests that people are motivated to seek social equity in the rewards they receive for high performance. According to him the outcome from job includes; pay recognition, promotion, social relationship and intrinsic reward. To get these rewards various inputs needs to be employed by the employees to the job as time, experience, efforts, education and loyalty.He suggests that, people tend to view their outcomes and inputs as a ratio and then compare these ratios with others and turn to become motivated if this ratio is high. Leadership Effectiveness and Job Performance Leadership, or more specifically effective leadership, is every bit as crucial (if not more so) in African Organizations as it is throughout the world. African organizations are no different from others Worldwide in terms of striving for performance in order to be globally competitive. The South African situation, however, is incredibly complex as many organizations are caught in the middle of a web of authoritarian hierarchies and traditional leadership approaches, as well as bureaucratic hierarchies mixed with modern approaches to leadership * (Grobler, Wà ¤rnich, Carrell, Elbert and Hatfield, 2002). It is argued that effective leadership has a positive influence on the performance of organizations (Maritz, 1995; Bass, 1997; Charlton, 2000). Ultimately it is the performance of many Individuals that culminates in the performance of the organization, or in the achievement of organizational goals. Effective leadership is  instrumental in ensuring organizational performance. * (Cummings and Schwab, 1973; Hellriegel, Jackson, Slocum, Staude, Amos, Klopper, Louw and Oosthuizen, 2004). As a result, many leadership theories have been proposed in the last fifty years which are claimed to have influenced the overall effectiveness of the organizations where they have been employed. In the competitive world business environment it is vital that organisations employ leadership styles that enable organisations to survive in a dynamic environment. * (Maritz, 1995; Bass,1997) Performance has been defined by Hellriegel, Jackson and Slocum (1999) as the level of an individual’s work achievement after having exerted effort. * Cummings and Schwab (1973) and Whittenand Cameron (1998) believe that performance is ultimately an individual phenomenon with environmental variables influencing performance primarily through their effect on the individual determinants of performance – ability and motivation. * Behling and McFillen (1996) confirmed the link between high performance and leadership in the United States by developing a model of charismatic/transformational leadership where the leaders’ behaviour is said to give rise to inspiration, awe and empowerment in his subordinates, resulting in exceptionally high effort, exceptionally high commitment and willingness to take risks. It has been widely accepted that effective organizations require effective leadership, and organizational performance will suffer in direct proportion to the neglect of this (Maritz, 1995; Ristow, Amos and Staude, 1999).

Annotated Bibliography Essay Example | Topics and Well Written Essays - 1250 words - 4

Annotated Bibliography - Essay Example ents disagree that the Direct Method is the most appropriate technique and 86% do agree that carefully planned translation activity can play a useful role in the L2 communicative classroom†¦Ã¢â‚¬  (Druce, 2012, p.1). The information created in the journal article provides much information to teachers. Teachers are required to enable students to acquire a second language with much ease. The article introduces attitude management and translation. In interpreting this article, one may point out that teachers are required to enable students develop positive attitudes towards their second language acquisition experience. It may be made successful by putting in place mechanisms that recognize social and cultural fluency. However, the recommended strategies may have numerous limitations. One limitation is that translation may hinder the effectiveness at which a student develops fluency in the new acquired language. This assumption hinders the credibility of the article. The article has presented my idea on teaching on second language acquisitions. Attitude development in the classroom setting has been cited as a requirement that was not considered in prior mechanism of teaching. Second language acquisition is based on the ability of a person to acquire an internal memorization of a new language (Donesch-Jezo, 2011). The main argument created by the article is that input and output of language properties are mandatory for a person to acquire a new language. The article is of the assumption that tutors are usually keen to provide input and not vigilant to monitor the output by students. The article addresses language output as the main topic. The article asserts that, â€Å"†¦another component which is required for successful second language learning is output†¦Ã¢â‚¬  (Donesch-Jezo, 2011, p.11). In addition, in order for a tutor to teach a new language, they must understand how it is acquired by the learners. In regards to relevance, the article introduces an important aspect

A contevrsary about social, political , and cultural impacts of Research Paper - 1

A contevrsary about social, political , and cultural impacts of science and technology on socity - Research Paper Example Most of the traditional studies are still focusing narrowly on how to make new things rather than whether these innovations are desirable or necessary to the society (Bell, 2006). This paper purports to discuss the political, social, and cultural impacts of science and technology to the society. Political impacts do with the mode in which science and technology advancement influences policies and policy-makers (Zeleza, 2004). This includes the attitudes and interests of administrators, politicians, and the public towards an issue of community interest towards science and technology, the actions of the public, and individuals’ involvement in science and technology solutions (Bulger, 2005). We are currently living in a digital world whereby science and technology advancement is changing processing, ownership, and usage of information. This revolution causes both positive and negative impacts to the political status of many countries. The key to success of any country’s democracy is a well-informed public. Science and technology advancement has improved the nature of intellectual property rights and that of copyrights (Zeleza, 2004). In future, intellectual property rights’ harmonization and biodiversity conservation are going to improve due to technologic al advancement. The fundamental political development in most countries is highly influenced by science and technology advancement since governments are able to distribute political, social, and economical information to its society (Cutcliffe, 2007). In order for a country to experience a friendly democracy, the society must have full access to basic economic and political information. Advancement of technology such as social media networks enables people to share political information and ideas with other people (Bulger, 2005). Science and technology advancement is making it easier and possible to distribute political information beyond place and time reaching to a

Key Strategic Alliances in Middle East Assignment

Key Strategic Alliances in Middle East - Assignment Example We should study about the Middle East because we can learn from the trials and tribulations suffered over the last few thousand years. In addition to this, the Middle East is at the center of three continents: Asia, Europe, and Africa. This makes not only the land valuable, but also the trade routes that go through this region. This perhaps explains why there have been so many wars in the region and why there is still conflict there today. Middle Eastern culture has also greatly influenced the way that westerners think, such as through mathematics. The origin of western numbers is Arabic, and many mathematical concepts first originated in the Middle East. Looking towards the future, the situation in the Middle East is changing as local economies experience growth and the people want to have more of a say in the political process. Learning from these experiences can only help understand the western form of democracy.

Causes of building collapes Term Paper Example | Topics and Well Written Essays - 250 words - 2

Causes of building collapes - Term Paper Example This means that as long as there are natural disasters, the risk of building collapse will continue to be recorded. This point is made against the backdrop that in most cases of natural disasters such as earthquakes and tsunamis, almost all types of buildings collapse including short buildings and skyscrapers alike. What this teaches is that natural disasters perhaps have the worse effect or impact on building collapse. Having said this, it would also be appreciated that even when an ordinary building and skyscraper all fall, the impact that will be created in the two can never be said to be the same. This is because the extent of loss that will be recorded with the falling of a skyscraper in terms of lives and property will certainly be more than what will be recorded with a shorter building. What this teaches is that as far as natural disasters as cause of building collapse are concerned, it is important to ensure that areas that are noted to be disaster prone will not be clustered with skyscrapers and other tall buildings. Using the 27 February 2010 Chile tsunami as an example, Palermo et al (2013) made a new revelation on the extent of resilience that different buildings endure in the case of natural disasters such as tsunamis. The outcome of the tsunami revealed that there are two major types of buildings which are engineered buildings and non-engineered buildings. These categories are found in both high-rise buildings and shorter buildings. In the case of engineered residential buildings, natural disasters such as tsunamis are found to have very minimal impact. This is because such modern engineered buildings generally exhibit high levels of resilience to the disasters, being affected only in areas of non-structural components. On the other hand, not the same can be said of non-engineered buildings, especially residential buildings. This is because with such buildings, the impacts of natural disasters are not only experienced with

IDS Alert Essay Example | Topics and Well Written Essays - 500 words

IDS Alert - Essay Example If my computer pointer starts moving by itself then I will at once pull out the network cable (LAN) or phone line from my PC as it is the point through which any external source can enter my PC. Port 3389 is a remote desktop connection port in windows which is usually opened in many computers on network. (Denning, 1990) A hacker can exploit it using a remote desktop connection and could send malicious threats or Trojans to gain access to the computer from this port. Removing my PC from the network will help isolate it and stop transferring the threat or virus to other Computers on the network. A well define thought and planning is necessary before installing an Intrusion Detection System (IDS) on a network. Besides technical issues and product selection another serious factor are the resource issues that includes manning the sensor feeds, product expenses and infrastructure support that must also be kept in mind. After the computer has been isolated the next step would be to detect and remove the threat using the appropriate Intrusion Detection Systems. Updated versions of spy ware and Antivirus software will be installed on the PC if the current versions could not detect the threat.

Islamic Banking and capitalism Research Paper Example | Topics and Well Written Essays - 2000 words

Islamic Banking and capitalism - Research Paper Example First, globalization is commonly seen as an integration of developed and less-developed countries leading to a homogenization of sorts. For instance, the emergence of a middle-class in China and India is often mentioned as an example of globalization homogenizing the world, as is the global reach of brands such as Nike and Ford. Globalization has also lead to the increasing mobility of the population. This in turn, has sometimes not created homogenization, but differentiation. An example of this would be the development of Islamic finance—financial organizations that are based on Islamic principles rather than capitalist or Western financial models. Analysts generally identify the first of these as â€Å"a savings bank based on profit-sharing in the Egyptian town of Mit Ghamr in 1963.† (Ariff, 1988, p. 42) Many significant Islamic financial institutions then developed in the 1970s such as the Dubai Islamic Bank, the Faisal Islamic Bank of Sudan and the Baharain Islamic Bank. More recently, with the widespread immigration of Muslims to the United States banks such as the Devon Bank in Chicago have developed. Its website describes it in this way: â€Å"Devon Bank offers Islamic financing services designed to avoid conventional interest common in traditional banking products.† (http://www.devonbank.com/Islamic/) The emergence of Islamic financial services corporations is an example of globalization leading to leading to more differentiation rather than homogenization. Throughout the world, and particularly in the United States, it is a new and a different development for there to be financial institutions that offer financial services based on Sharia law (and of course compliant with the legal requirements of their host countries. Devon Bank makes this plain on its homepage: â€Å"Our products are created to meet your needs by a staff versed in both U.S. and Islamic legal requirements, and then submitted

Is Google Making Us Stupid Essay Example | Topics and Well Written Essays - 1750 words

Is Google Making Us Stupid - Essay Example I tend to differ with the notion of stupidity arising from Google. Although Google and the internet as a whole are changing us, we are headed for good things. Carr claimed the emergence of the search engine Google carries blame for his acquired inability to pay attention to whatever material he reads. Google provides information within a span of 0.003 seconds and internet users find no use in reading large volumes of books to get information that is already summarized elsewhere (Carr 56). The summaries and appealing presentation of information over the internet are Carrs argument on his reduced attention and inability to read long texts and passages. It is, therefore, apparent that for people who use the internet and its search engines such as Google, they cannot read articles and books with competency. They also cannot take much of their time trying to comprehend what they read (Carr 56-63). Contrary to that, optimistic people will always quote how Google has shaped and reshaped their minds and brains. Google has provided people with means, new tools and ways of accessing information and sharing it with the rest of the world. It, therefo re, sounds absurd to think of Google as having a stupid nature rather than enlightening tool. It is unacceptable to take Google as a tool for stupidity. People’s failure to pay attention for long texts has been depleting over time because of the corrupt cultures present in the world (Gitlin 5-7). James Bowman in his article "is stupid making us Google" expounds on the concept that Google takes no fault on the new state of poor attention people are paying to read hard copy materials. Instead, he shifts the blame to the junk culture blending all over the world. Education instructors and mentors, for example, have reduced their attention and value for their respective studies (Bowman 75-78). Bowman goes ahead to elaborate his claims by

Design and Build of Movable Chair For Handicapped Hunters Assignment - 2

Design and Build of Movable Chair For Handicapped Hunters - Assignment Example Odland and Schafer modeled the joystick control which moves the gun or bows up and down or side to side after the joystick found on modern wheelchairs. There is a tiny camera mounted behind the scope of the gun which projects the image of crosshairs and the target onto a video monitor the hunters can easily view from their wheelchairs† (Yankovich, 2010, par. 6). The author is actually an entrepreneur who owns a jewelry shop in Lowell, Michigan. He had first-hand access to the information knowing Pete Odland personally from their common demographic location. The article provided relevant information as a unique and useful design to assist handicapped hunters in regaining mobility to enjoy what they love most. The innovative design was able to help hunters regain â€Å"the freedom for physically challenged people to â€Å"do it themselves†. The very design puts control back into the hands of those who feel they have lost any vestige of control over their lives† (Yankovich, 2010, par. 14). The article provided information on the quad chair designed by Steve Peeples initially for Aaron McMikelk, a handicapped hunter who lost the use of his legs due to viral infection. As indicated by the author, â€Å"Peeples purchased a used wheelchair, removed all the parts, such as brakes and wheels, and mounted the apparatus on the front of his quad runner. His hope was that his "Quad Chair" would open a whole new opportunity for people with limitations† (Ottesen, 2009, par. 13). The innovative way to mount a disabled chair to a vehicle enabled ex-Marine McMikelk to enjoy hunting despite immobility. The author is an outdoor columnist who is on the lookout for stories that would inspire readers to enjoy outdoor activities. As indicated, â€Å"with less than $200 needed to outfit his quad, Peeples hopes his idea will catch on around the country with veterans and handicapped shooters,  and those fewer mobiles† (Ottesen, 2009, par. 17).

Carrying a Concealed Handgun Essay Example for Free

Carrying a Concealed Handgun Essay Carrying a concealed handgun is widely argued. It is legal in 49 states. Many people think carrying a gun will increase crime and start public shootouts. This is not the case. Carrying a concealed handgun is okay. It is also a right protected by the Constitution. In District of Columbia v. Heller (2008), the Supreme Court ruled that the Second Amendment protects â€Å"the individual right to possess and carry weapons in case of confrontation. This meaning is strongly confirmed by the historical background of the Second Amendment. † Handguns aren’t given to just anyone that walks in. The Gun Control Act of 1968 limits who can receive a handgun. Criminals cannot receive permits to carry a concealed handgun. Law enforcement agencies do background checks to ensure they are allowing the right people to carry a handgun. The mentally ill are also included in the Gun Control Act. If someone commits a crime with a legal carry permit, they would do it whether the handgun was legal or not. This doesn’t happen often though. According to ConcealedGuns.ProCon.org, only .003 % of murders between May 2007 and March 2010 were committed by handgun permit holders. The website also shows that the â€Å"general public is 5.7 times more likely to be arrested for violent offenses and 13.5 times more likely to be arrested for non-violent offenses, than concealed carry weapon permit holders.† Concealed handgun permit holders are, for the most part, good people. Some do lose their permits but permit revocation rates are very low. The Wisconsin Policy Research Institute Report, Concealed Carry Legislation, shares this data: -In 2004, Utah had a permit revocation rate of .4% -From 1986-2003, Kentucky had a permit revocation rate of .8% -In 2001, Indiana had a permit revocation rate of .2% -From 1995 to 2005, NorthCarolina had a permit revocation rate of .1% -From 1995-2006, Virginia had a permit revocation rate of .2% -From 1994-1996, Wyoming had a permit revocation rate of .2% -From 1996-1999, Oklahoma had a permit revocation rate of .2% This data clearly shows that only a very small group of people do something to have their permit revoked. It also means that a vast majority of permit holders are responsible, law abiding citizens. The handgun itself doesn’t have to do the defending. If a criminal knows that individuals can carry handguns, they are less likely to attack, which helps the public as a whole. If someone carrying a handgun and is attacked, the attacker usually retreats when the handgun is drawn. The police do not have to protect us. According to www.nraila.org, the Warren v. District of Columbia (1981) court ruled that â€Å"official police personnel and the government employing them are not generally liable to victims of criminal acts for failure to provide adequate police protection . . . a government and its agents are under no general duty to provide public services, such as police protection, to any particular citizen.† Also, the court ruled in Bowers v. DeVito (1982) that â€Å"[T]here is no constitutional right to be protected by the state against being murdered by criminals or madmen.† The government cannot guarantee the safety of everyone. Personal protection is important. Carrying a handgun also makes the carrier feel safe. Americans should not be scared going outside at night or walking through certain neighborhoods. Concealed handguns could also aid in public shooting sprees. If the individuals in the Virginia Tech or any other public shooting massacre had been armed, the spree could have ended much sooner. Some believe the massacre would have been made worse but what could be worse than a 32-victim massacre? Most of the victims were of legal age to possess a firearm. If they had been allowed a handgun on campus, could lives have been saved? I strongly believe so. With that said, this doesn’t mean people should run around as vigilantes hunting down criminals. The concealed carry permit holders are not the police. They do not have the authority to shoot just anyone. There are laws that make sure this doesn’t happen. Handguns would just be used for defense. Although you can’t run around fighting crime, your situational awareness while carrying a handgun will go up. You know that you are carrying and anyone else can be carrying too. This situational awareness can help you deal with bad situations or even prevent them. How do schools fit into this? Legally, a person with a concealed handgun cannot be within one thousand feet of a K-12 school. For the most part, concealed weapons are not allowed on college campuses either. This creates a large upset in the college community. Very few colleges have allowed students to carry concealed weapons. There have been no problems. Twelve schools, from Virginia, Colorado, and Utah, have allowed students with handgun permits to carry them on campus. Not one of these schools has had a handgun-related problem. This includes murder, suicide, threats, and handgun theft. Some believe that carrying handguns will distract students from the educational environment. These handguns are concealed. People should not know if you are carrying one or not. The students aren’t distracted in their daily lives by handgun permit holders so why should the college campus be any different? The same goes for the argument that guns and crowded schools don’t mix. Public areas are crowded too. College students are adults. A twenty one year old who is not in college can have a concealed handgun so why can’t an individual who is in college have one? They know what they are doing. They still have to follow the handgun laws like any other permit holder. College life may be defined with alcohol and drugs but the law doesn’t make exceptions for college parties. If an individual is intoxicated, they cannot be carrying their weapon. In the end, handguns are not a bad thing to carry. They can protect you in many ways. Crime rates may drop if criminals are too scared to attack. Carrying a concealed handgun is a right given to Americans. It should not be wasted. Almost all legal permit holders are law abiding. Guns can help.

Eyesenck’s Personality Theory Model: Sanguine Personality

Eyesenck’s Personality Theory Model: Sanguine Personality Introduction Eysenck’s Personality Theory Model Eysencks personality of theory divided into two categories which are extraversion and introversion personality types. Extraversion type of personality person is usually relax and confident on their own while introversion type of personality person is often a quite person; focus on inner feelings and their emotional will be more negative and not stable as compared to extraversion type of person. Extrovert people are more likely to be out-going person, they like to social and attend event to meet with new people and make new friends. In contrast, introvert people keep things to their own and seldom share to people, they also less likely towards social things because of their shy personality. In this world, different types of people having different own personality, by understanding people’s personality is a key to unlock elusive human qualities. All humans are different, everyone has their own value, principles, special strengths, weakness and qualities in handling things in their life; different types of people will think and action in different ways. For instance, Eysenck’s categorize people into 4 types of human personality, which are choleric, melancholic, sanguine and phlegmatic. However, choleric and sanguine personality were fall under extraversion category while melancholic and phlegmatic were fall under introversion category. Features of Sanguine Personality Referred to Eyesenck’s Personality Theory Model, sanguine personality is playing a role as the extrovert, talker and optimist. They are emotionally stable compared to people who have melancholic and choleric personality. The characteristics of sanguine personality are sociable, outgoing, lively, easygoing, talkative, responsive, leadership and carefree. Sociable People who have sanguine personality are sociablen; they are supportive in engaging themselves in outside activities and talk to others. Sanguine people have a lot of friends and also have wide social relationship which may have friends that are from different fields or places. Besides, they can make new friends easily and adapt to the situation even in a new environment or with strangers. Outgoing People who having sanguine personality is an outgoing person; they are active in joining or participating outside activities. Sanguine people don’t like to stay at home and things that are boring. They like to join or see thing that are challenges and new and they are type of person who enjoy going out or hang out with friends and comfortable in social situation. Lively People who having sanguine personality is a lively person; they are active at most of the time and having positive energy in whatever things that they do. Besides, they bring fun and joy to people surrounded them and people will feeling comfortable and relax when with sanguine people as they less likely having negative thought compared to melancholic personality. This may one of the features of sanguine that attracted people to talk and make friends with them. Easygoing People who having sanguine personality is an easygoing person; they forgive and forgot mistake or bad thing that people done on them. They don’t hold grudges and usually behave in a relaxed and tolerate approach or manner. They have even-tempered and able to control their own emotional compared to melancholic and choleric personality. They seldom mad at people and don’t like to make things become complicated. For instance, if sanguine people commit a mistake they will readily apologize to the person. Talkative Sanguine playing roles as the talker and storyteller, they are talkative and like to repeat stories to their friends. They can talk anything with their friends; such as gossip or discuss things that from general problems until privacy with their friends without feeling tired or bored. They eager to express their own opinion or idea, wish people paid attention on them. Sometimes, due to the talkative personality they are likely to dominate the whole conversation when in a group activities. Responsive Sanguine people are responsive; they give quick and readily response to people. They are open to suggestions; accept people comment and improvement themselves from it. Besides, sanguine people usually are the type of active students in the class; they like to ask and also give response to their teacher questions. If sanguine friends are facing problems, they will response to it and always be there to listen and to give opinion or advice to their friends. Leadership Sanguine people tend to have strong leadership; they have the ability to guide a group of people and give direction to the members about actions that should taken in order to achieve a certain task. Sanguine people like to express their own opinion and ideas in group activities and sometimes they will be the dominant person throughout the whole conversation with their friends or activities. In a group, sanguine will act as opinion leader and they have the power to influence or swing people’s perception and action. Carefree People who having sanguine personality is a carefree person, they are relaxed and less worried. For instance, sanguine people behaving in a happy-go-lucky attitude, when everyone was stressing and rushing for group assignment, sanguine may be one of the most relaxed people among the group members. They are less likely to take thing seriously, free from care and they always enjoy their time in a happy and joy mood. Communication Style The communication style of sanguine personality is talkative. People who having sanguine personality are like to talk, they can talk anything from general problems until privacy without feeling tired or bored. Sanguine people talk in confident and relaxed manners; they talk things in more casual way and always share a lot of things with their friends. They like to repeat stories, always response to people questions and give comment or opinion. Their communication style will attract people attention and people will feel comfortable when communicate and interact with sanguine people. However, due to the talkative personality, sanguine people always talk and rush for conclusion. Sometimes, they didn’t give opportunity for others people to speak or express their opinion. Responsive: â€Å"My friend, I am here listening to your problems.† Carefree: â€Å"It is okay, don’t worry so much and everything will be alright.† Each personality has their pros and cons. The strengths of sanguine personality are they are an easygoing and sociable person. In contrast, the weaknesses of sanguine personality are they have a strong leadership and talkative. Strengths: Sanguine is an easygoing person, they do not hold grudges. They forgive and forgot people mistake. They have even-temper and able to control their emotion, which make people like to make friends with them. It is because sanguine people will not mad at people easily or for a long time. People will feel relax and comfortable when with sanguine people because of their easygoing personality. For instance, even thought if a person has done something bad to sanguine people, sanguine people will readily forgive the person if they apologize or admit the fault. Besides, sanguine people do not mind share their things to their friends such as foods or ideas. They are also open in accepting people judgement, if people commented something bad about them they will not keep it in heart for a long time but will takes it as opportunity for self-improvement. Sanguine is a sociable person, by having this characteristic it will help them easy to get along with others and also help to encourage effective relationship with outsider. Sanguine people will be having a lot of advantages in their life by having sociable personality such as in workplace, school and others. Sanguine person is likely to engage themselves in outside activities, they are active in joining any events which can helps them to extend their social relationship and make new friends. When they go out and know more friends, they actually can learn extra knowledge from other persons and also enhance interaction with other people. This could be a good opportunity for their education or business to go further, the chances that they get might be a good turning point for their life. For example, sanguine person looking for a job after graduation, they might will get job more easily than their friends as they have knew different friends that worked in different fields, it is way ea sier to approach someone when good relationship is established. Weaknesses: By having strong leadership personality it might be one of the weaknesses of sanguine people. People who are having strong leadership is usually self-centred, they want people to paid attention on them and wish to be dominant in group activities. They are eager to express their ideas and opinion, they hope whatever they said can be heard or followed by members. However, this personality will cause distancing between sanguine people and their friends. It is because to some extend leader having the power to instruct members to do something, for those people who are not obedience they will surely don’t like these type of person. Sometimes, sanguine person are assertive, they might over confident on their own and like to showing off and self-promoting. They are likely to take the full responsibilities as a leader when there is any job distributed among the members, due to the strong leadership personality Another weakness of sanguine personality is talkative. Sanguine people are likely to repeat or talk about the same things to their friends without feeling bored or tired. Due to talkative personality, sometimes people that friend with sanguine people may feel they are annoying For example, when sanguine people found that they are not satisfy with what they had they will keep asking and finding until they found out the satisfy answer or result. They need response and attention from others. They talk in a casual or fun way people will think that they are not taking things seriously. Due to the talkative personality, people will feel that they are exaggerating as sanguine people will give excessive information or overselling ideas when communicating to other people. Recommendations There are several ways to overcome the weaknesses of sanguine personality. First, sanguine person should learn to listen to others people words before they speak or express their view or ideas. In doing this, they can give opportunities for other people to express their ideas and sometimes the ideas given by people may be useful in their group activities instead of dominate the whole conversation. Second, they should learn to self-effacing which is to tone down their strong approach, avoid exaggerating and overselling own idea as it may help to gain more trust from friends toward them. Third, in whatever thing that sanguine people do they should always put themselves in other people shoes, should consider about other people interest and care more about other people feeling. Besides, it is always important to consider the seriousness of the situation first but not behaving in a carefree attitude at all the time. References Coon, D Mitterner, J.O. (2010). Introduction to psychology: Gateway to mind and behaviour, 12th ed. California: Thomas Wadsworth. Pastorino, E.E. Portillo, S.M.D. (2012). What is psychology. 2nd ed. Belmont: Thomson Wadsworth. Delamater, J.D. Myers, D.J. (2010). Social psychology. 7th ed. CA: Thomson Wadsworth.

Mutant and Wild-type Yeast Strains via Mitochondria Proteins

Mutant and Wild-type Yeast Strains via Mitochondria Proteins Differentiating between mutant and wild-type yeast strains via mitochondria proteins    By: Jason Hoang Lab Partner: Daryan Chan Introduction Yeasts are important organisms due to their uses in everyday such as baking, making fermented foods and alcohol production (Steensels et al, 2014). Yeasts have been so widely studied that it was one of the first organisms to have its genome sequenced (Goffeau et al, 1996). Thus, Yeasts are more than capable of acting as a model organism for eukaryotes (Botstein et al, 2011). For this experiment we are working with Saccharomyces cerevisiae. The mitochondria is the powerhouse for cell, as it is the major production site of ATP for the cell. The inner mitochondrial space has an electrochemical gradient, from which ATP is generated by using 5 protein complexes create an electrochemical gradient to assist in ATP production (Alberts et al., 2015). The COX6 assembly accepts electrons from cytochrome c and uses oxygen as the terminal electron acceptor to make water (Alberts et al., 2015). ATP synthase then uses the resulting proton gradient made by those complexes to pump protons back into the mitochondria matrix and make ATP (Alberts et al., 2015). The objective of this experiment was to determine if a given yeast sample was a wild type or a mutant with no COX6 activity. One of the major techniques to be used in this lab is subcellular fractionation. This technique first lyses the cells and then uses centrifugal forces to separate particles by size (Alberts et al., 2015). The centrifugal forces results in the denser particles moving away from axis of rotation creating a pellet which contains the heavier particles and a supernatant which contains lighter particles (Alberts et al., 2015). Another major technique used was Gel electrophoresis. Gel electrophoresis is used so that a fraction with multiple proteins can be separated based on size and shape (Alberts et al., 2015). Protein fractions are loaded on to wells in the gel and an electrode is attached (Alberts et al., 2015). SDS page is popularly used because it can confer a negative charge and linearize proteins being run through the gel (Alberts et al., 2015). The proteins will run through the gel due to their negative charge (Alberts et al., 2015). A standard is used to provide a reference to determine the sizes of the sampl proteins (Alberts et al., 2015). One of the other major techniques used in this lab was western blotting. After proteins are run on electrophoresis a labelled antibody is exposed to the electrophoresed fractions in a process called immunoblotting in order to detect presence of a specific protein (Alberts et al., 2015). The gel is exposed to a membrane where a current is run to dive the proteins onto the membrane (Alberts et al., 2015). The membrane is then drenched in labelled antibodies to detect for a specific protein (Alberts et al., 2015). This process can detect very small amount of specific protein and is useful for detecting changes of concentration of a specific protein in a cell under various conditions (Alberts et al., 2015). To measure cytochrome oxidase activity in this lab, we looked towards Beers Law which states that the ability of a solution to absorb light at a single wavelength is proportional to the concentration of solute in solution (Lukofsky et al, 2009). This show that absorbance and concentration are linearly related. Therefore, this would allow us to determine the rate of cytochrome oxidase activity in a sample. Materials and Methods Experiment was performed according to protocols set by Department if Biology, Winter 2016, Biology 331 for Experiment 1: Subcellular fractionation of yeast cells, pg 2-8, Experiment 2: Yeast growth curve; light microscopy; protein determination, pg 1-4, Experiment 3: Polyacrylamide gel electrophoresis, pg 1-8, Experiment 4: Development of Western Blot; COX Activity Assay, pg 3-10, written by Dr. Dragana Miskovic where the experiment was performed with no deviations unless specifically noted (Miskovic, 2017) The only deviation occurred in experiment 2 where we had ran out of BSA STD and had to borrow from another group. The borrowed BSA STD was not tested to have exact concentration as specific in lab protocol and may have had different concentration. Results Experiment 1 Yeast Strain Sample: A2 Table 1. Masses recorded for Lysing Yeast Cells Section Items Mass (g) Mass of Centrifuge Bottle containing Yeast 159.2 Mass of Empty Centrifuge with Pellet 49.89 Mass of Pellet 3.23 Amount of STE solution needed to resuspend yeast pellet: 3.23g x 2= 6.46mL Table 2. Volumes recorded for the Aliquoting Yeast Subcellular Fractions Section Solutions Total Volume LPS 3mL + 3.7mL= 6.7mL HSS 3mL + 2.8mL= 5.8mL MITO 300ÃŽÂ ¼L + 300ÃŽÂ ¼L= 600ÃŽÂ ¼L It was also noted that after the MITO fraction was made the pellet was intact and not messy Figure 1: Drawings of 50/100/200 ÃŽÂ ¼L dye drops from pipetman and expected 1 mL dye drop Experiment 2 Part A Table 3. Concentration of Yeast Cell at Two Different Times Time OD600 Reading Concentration (cells/ mL) 2:47pm 0.021A 210,000 4:59pm 0.043 A 430,000 It has been determined that an OD600 value of 1.0 is thought to contain roughly 1 x 107 cells/ mL. An OD600 value of 0.021 A will contain a concentration of 210 000 cells/ mL. An OD600 value of 0.043 A will contain a concentration of 430 000 cells/ mL. Figure 2. This graph shows the change in absorbance of yeast culture at 0 and 120 minutes. Equation to represent growth is calculated and shown above. Calculating Doubling time Formula for growth of yeast is y=0.0002x + 0.021. given initial absorbance reading of 0.021, doubled concentration should give reading of 0.042. Therefore use y= 0.042, where x means time in minutes 0.042=0.0002x + 0.021 where x = 105. Therefore it was found that doubling time is 105 minutes. Part B Yeast cells dyed with methylene blue stain Figure 3. These are some of the cell types that were observed when the overnight culture was stained with methylene blue under 40x magnification. It was found that roughly a third had a stained positive for a nucleus. None of the cells appear to be multi-nucleate. No vacuoles were observe either Yeast cells dyed with neutral red Figure 4. These are some of the cell types that were observed when the overnight culture was stained with neutral red under 40x magnification It was found that over 90% of the cells stained positive for a nucleus. Many of the cells appeared to be multi-nucleate and budding as well. It appeared that 1 or 2 vacuoles appeared to be detected per cell. Part C Table 3. Absorbance Results for the BioRad Protein Determination Assay 1 2 3 4 5 6 7 8 9 10 A 0.27 0.191 0.21 0.201 0.196 0.37 0.397 0.404 0.369 0.036 B 0.246 0.24 0.303 0.192 0.226 0.245 0.272 0.372 0.252 0.035 C 0.23 0.263 0.248 0.294 0.037 0.036 0.036 0.036 0.036 0.036 D 0.256 0.227 0.25 0.277 0.035 0.035 0.035 0.035 0.035 0.034 E 0.246 0.182 0.242 0.215 0.474 0.362 0.306 0.389 0.482 0.035 F 0.289 0.349 0.285 0.246 0.299 0.264 0.347 0.738 0.203 0.036 G 0.203 0.254 0.321 0.249 0.035 0.035 0.035 0.037 0.035 0.036 H 0.2 0.261 0.263 0.274 0.034 0.034 0.035 0.035 0.035 0.034 11 12 A 0.036 0.037 B 0.035 0.035 C 0.037 0.038 D 0.035 0.044 E 0.036 0.035 F 0.035 0.035 G 0.035 0.035 H 0.035 0.034 Figure 4. This graph shows absorbance readings of standard solution using BSA at different concentrations. Calculating concentrations of LSP, HSS, and Mito. Equation for concentration of solution based on absorbance reading was determined based on above graph. Equation yielded was y=0.1264x + 0.2159 Average absorbance readings: LSP was 0.245, HSS was 0.249, and MITO 0.289. Using found readings as y for above equation we calculated concentration of proteins in each sample. LSP: 0.245 = 0.1264x + 0.2159Therefore x = 0.230 mg/mL HSS: 0.249 = 0.1264x + 0.2159Therefore x = 0.262 mg/mL MITO: 0.289 = 0.1264x + 0.2159Therefore x = 0.551 mg/mL Dilution factor needed to get fraction to 2ÃŽÂ ¼g/mL LSP: (0.230 mg/mL)(0.1mL)=> (0.0230 mL)(1/x mL)(10 dilution factor) = 2mg/mLTherefore x= 0.115 mL HSS: (0.262 mg/mL)(0.1mL)=> (0.0262ÃŽÂ ¼g)(1/x mL) (10 dilution factor) = 2mg/ mL Therefore x= 0.131 mL MITO: (0.551 mg/mL)(0.1mL)=> (0.0551ÃŽÂ ¼g)(1/x mL) (10 dilution factor) = 2mg/ mLTherefore x = 0.2755 mL Experiment 3 Figure 5. PVDF Membrane after proteins are transferred over from gel after electrophoresis. Our group is left side (D.C, J.H) Experiment 4 Figure 6. Membrane after detecting solution had been added over 10 minutes ago. Bands on right hand side are the standard Figure 7. This graph shows the distance travelled by each protein in the standard mix against the Log(Mw) on semi log paper Table 4. Cytochrome c Oxidase (COX) Activity Assay Sample Absorbance At 0 sec (OD) Absorbance after 20 sec (OD) Change in absorbance Change in Concentration (ÃŽÂ ¼mol/mL) COX activity (ÃŽÂ ¼mol/ L/min) Blank 0.525 0.525 0 0 0 LSP (1) 1.259 1.253 0.006 0.2143 0.6429 LSP (2) 1.272 1.264 0.008 0.2857 0.8571 HSS (1) 0.493 0.491 0.002 0.0714 0.2143 HSS (2) 0.496 0.491 0.005 0.1786 0.5257 MITO (1) 0.553 0.557 -0.004 -0.1429 -0.4286 MITO (2) 0.537 0.535 0.002 0.0714 0.2143 Table 4. This shows the Sample calculation for COX activity Change in absorbance = Absorbance at 0 sec Absorbance after 20 sec 1.259-1.253 = 0.006 Change in concentration ΆA = ÃŽÂ µ x b x Άc 0.006 = 28mM-1-cm-1 x 1 cm x ΆcTherefore Άc = 0.0002143 mM => 0.2143 ÃŽÂ ¼molAssuming volume of assay is 1.0 mL, change in concentration is 0.2143 ÃŽÂ ¼mol/mL COX activity COX activity = change in concentration / time 0.2143 ÃŽÂ ¼mol/mL / (1/3 min) = 0.6429 ÃŽÂ ¼mol/mL/min Figure 8. Graphical representation of COX activity in LSP fractions Figure 9. Graphical representation of COX activity in HSS fractions Figure 10. Graphical representation of COX activity in MITO fractions Discussion Galactose was used over glucose as a carbon source for our yeast cells. This is because we wanted to determine if the mitochondria was functional in our yeast cells. Different yeast strains will use different metabolic pathways in presences of each. When glucose is used as a carbon source the yeast cells will generate ATP via fermentation, whereas when Galactose is used the cell will perform oxidation. This is important to observe as different yeast strains will have varying levels of cytochrome c usage based on that. To visually determine if cytochrome c will be utilized by the cell we can look at the fractionation experiment earlier. When separating for the MITO fraction if one had found a messy pellet it would have indicated that the mitochondria was not intact while solid pellets would indicate the mitochondria was intact. If one did find a messy pellet it could have been the result of differences in fractionation techniques, cells being lysed prior, or something had disturbed the cell in transport. For our experiment we had found the mitochondria to be intact, which is a strong indicator that the mitochondria for our sample was present. To reach cytochrome c oxidase (COX) we used diferential centrifugation which seperates objects based on size and density, where larger molecules such as the intact cells will settle at the bottom of a tube while mitochondria which is smaller would remain in supernatant. This is also why we had separate centrifugations, to get samples with intact cells and samples with intact mitochondria. Density gradient centrifugation is also a widley used technique that seperates based on density. In that case we would see multiple bands form in tubes with densest molecules gathering at the bottom and bands above it with less dense molecules. Experimentally we found yeast doubling time to be 105 minutes (1.75 hours) when inoculated in YPD (1% yeast extract, 1% peptone, 2% glucose). It has been determined in many other experiments that Saccharomyces cerivisiae has a doubling time of 1.69 hours (Deak, 2008). The difference could be attributed to many factors such as environment (amount of light, heat, and etc) and growth substrates used. But the difference is not very large and would still be considered to conform to literature results. During the methylene blue staining of yeast cells it was noted that roughly a third of yeast cells contained a nucleus but it did not seem to be multi nucleate. While the neutral red stains showed that many cells appeared to be budding with one or 2 vacuoles present per yeast cell. These findings fall in line with what is normally expected from yeast cells as they do have vacuoles in their cells (Armstrong, 2010). Furthermore results also fall in line with yeasts having nucleuses but not being multi nucleated (Roberts and Ganesan 1959) One thing that may have affected a major portion of the experiment was determining the concentration of each respective LSP, HSS, and MITO fraction and diluting it to 2mg/mL. it is important to note that during pipetting steps to get each sample that the suspensions be homogenous beforehand otherwise you may be taking up different components of the fraction and missing others depending on how deep the pipet was inserted. During the remainder of the experiment it was found that after gel transfer to PVDF membrane and during western blotting that very few to no proteins were showing up. If low concentration of protein was a factor then it would most likely be traced back to this step. Many reasons can be attributed to this for instance, poor pipetting technique, the fractions were not homogenized properly before pipetting or even the dilution factor could have been incorrect. As noted during the material and methods during the preparation of experimental samples which would to create o ur protein concentration standard curve we had run out of BSA STD and required taking some from another group. When we created our protein concentration standard curve it came out completely odd, having unexpected drops in absorbance readings. The expected result was a linear curve where a higher BSA STD concentration would have led to a higher absorbance readings. Due to the change in BSA STD this may have had a different concentration due to being taken from a different location in its container it could have had a different concentration. Thus causing inconsistencies for our standard curve. As the standard curve was deemed incorrect afterwards any protein concentration calculations based on it would have been flawed, leading to incorrect dilutions. If the dilutions been calculated incorrectly, as they most likely were, there is the chance that the protein fractions would have been over diluted leading to not enough protein to be present for visible bands for the gel electrophores is and western blotting. For the gel electrophoresis SDS was included in solubilisation buffer to give proteins inserted into the wells a negative charge so that when a charge was applied they would run to the other end of the gel and to help unfold the protein so that it would be able to go through the gel. For this experiment a 12% gel was used in the interest of saving time because a 15% gel would have caused the proteins to go through it slower leading to a lower resolution of identifiable protein bands. The purpose of transferring proteins from gel to PVDF membrane was to be able to visualize the movement of proteins on the gel after electrophoresis. To accomplish this we applied Ponceau stain to the membrane to increase the resolution of the bands and to ensure equal amounts of proteins are loaded onto the gel (Al-Amoudi et al., 2013). It was found that after proteins had been transferred to PVDF membrane that we had very few bands show up for the solubilizing buffer lane, both LSP sample lanes, and both HSS sample lanes. Bands did appear for both MITO samples, however, it appears that got smeared across the gel, bleeding over to other wells. This could have been the result of diluting samples in the wells for reasons noted above, the SDS gel would have been poorly constructed and contributed to the smearing, and poor electrode contact on the gel might have blocked the gel from having proper electrical charge. Issues could have also arisen during transfer of proteins from gel to me mbrane. Air bubbles could have been present during transfer which would have prevented any protein from being transferred as proteins cannot move through air. Additionally, poor folding between membrane and the gel could have attributed to smearing of MITO samples. The purpose of the western blotting was to be able specifically detect for the presence of biotinylated COX proteins. In order for a cell to express a biotinylated protein it needs to be able to take up foreign DNA, be able to properly fold COX-biotin fusion protein, the cell needs to be able to recognize the BSS signal fused to C terminus, and be able to translate COX and biotin together (moving stop codon so that it doesnt not stop halfway across the other.) It was found that after western blotting our membrane with protein fractions that no bands had appeared even after 10 minutes of membrane being in contact with detecting solution. This led to Figure 7. The chart showcasing the relative distances that proteins have travelled is blank as a result. This would imply that when the blocking solution was added that it managed to block the entire membrane (and any present proteins included) from interacting with the probe. However Tween-20 was used to wash excess reagent. So the milk most likely would not have been able to bind to any protein after introduction of Tween-20. Therefore the lack of data could be attributed to low concentrations of protein on membrane for reasons as noted above. Referring to Figure 5 the only proteins that were found on the membrane after were MITO which shows that there would have been no LSP or HSS for probe to bind to, whereas for present MITO sample the concentration may not have been high enough and as a re sult some of it could have been washed out by the methanol step causing concentration of MITO to be so low that it could have been blocked by the blocking solution. There is also a possibility that our yeast samples were not able to biotinylate the COX protein at all which could explain why there were no bands occurring Looking at COX activity graphs for LSP, HSS, and MITO they seem to follow what is expected except for HSS. COX was used as an identifying marker for identifying subcellular fractions containing COX because it is an integral membrane protein for the inner membrane space. If COX activity is present then that would indicate that the mitochondria is intact and functioning. These samples should have seen increased COX activity as cytochrome c was introduced into the fractions which provide electrons to the COX protein allowing it to pump proteins and reduce oxygen to water. Both MITO and LSP experienced increased COX activity as shown by figure 8 and 10 respectively. This falls in line with what was expected with the MITO fractions experiencing higher levels of COX activity then the rest as the cytochrome c had less of a distance to travel to reach inner mitochondrial membrane space than LSP. LSP should have a signal because it would contain intact yeast cells which have mitochondria Albe rts et al., 2015). Therefore LSPs rate of COX activity should be lower because the cytochrome c would have harder time reaching mitochondria. This is shown by Figure 10 having steeper reaction times than Figure 8 and 9. This reaction utilized Deoxycholate (DOC) to speed up the reaction which is why it was only done in 20 second intervals as DOC solubilizes with cytochrome c so that it can enter the mitochondria to interact with COX. If reactions were tested too long after DOC was added then the reaction would have finished before being able to measure absorbance. The one that did stand out was the HSS fraction which appeared to experience negative COX activity or none at all. This was expected as it should have all the remaining parts of the cell that werent the mitochondria, lysosomes and peroxisomes Alberts et al., 2015). This would indicate that these samples did not have an intact mitochondria with a COX protein to interact with cytochrome c. this could be explained by the In conclusion it was found that our yeast strain A2 is the wild type strain. This is because during initial centrifugation the resulting pellet was solid indicating intact mitochondria. Furthermore during COX assay the MITO strain indicated an active COX as shown by its increase in activity, proving that A2 was in fact a wild type strain with functioning mitochondria. References Al-Amoudi, M.S., Salman, M., Al-Majthoub, M.M., Adam,Abdel Majid A., Alshanbari, Naif A., Refat, Moamen S., (2013) Res Chem Intermed 41: 3089. doi:10.1007/s11164-013-1417-4 Alberts, B. et al. (2015). Molecular Biology of the Cell Sixth Edition. New York, NY: Garland  Science, Taylor Francis Group. Armstrong, John. Yeast vacuoles: more than a model lysosome. Trends in Cell Biology 20.10 (2010): 580-85. Web. 13 Mar. 2017. Botstein, D., Chervitz, S. A., Cherry, J. M. (1997, August 29). Yeast as a Model Organism. Retrieved March 12, 2017, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3039837/ Deak, Tibor. Handbook of Food Spoilage Yeasts, Second Edition. Contemporary Food Science (2007): 50-51. Web. 13 Mar. 2017. Glerum, Moira Miskovic, Dragana (2017). Biology 331 Advanced Cell Biology Laboratory Manual Winter 2016. Biology Department, University of Waterloo, Waterloo. Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD,  Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG  (1996) Life with 6000 genes. Science 274, 546 Lukofsky, David, Jonathan Bessette, Heejeong Jeong, Elsa Garmire, and Ulf ÃÆ'-sterberg. Can precursors improve the transmission of energy at optical frequencies? Journal of Modern Optics 56.9 (2009): 1083-090. Web. 13 Mar. 2017. Roberts, C., and A. T. Ganesan. The occurrence of multinucleate giant cells in yeasts. Antonie van Leeuwenhoek 25.1 (1959): 97-107. Web. 13 Mar. 2017. Steensels, Jan, Tim Snoek, Esther Meersman, Martina Picca Nicolino, Karin Voordeckers, and Kevin J. Verstrepen. Improving industrial yeast strains: exploiting natural and artificial diversity. FEMS Microbiology Reviews 38.5 (2014): 947-95. Web. 13 Mar. 2017.

Kate Chopins The Story of an Hour Essay -- Kate Chopin Story Hour Ess

Kate Chopin's "The Story of an Hour" In â€Å"The Story of an Hour† by Kate Chopin, the author depicts how someone can be trapped in an unproductive and unsatisfying reality because of other’s thoughtlessness, exploitation, and domination. When combined with the contemporary society’s belief, presumably the later half of the 19th century, a further understanding of Chopin’s thoughts and feelings can be realized. Mrs. Louise Mallard, the victim and messenger of this story, is the image of such a person. Her relationship with her husband is so oppressive and limiting that even death is considered a reasonable means of escape. The condition of life for Mrs. Mallard is terrible, yet for some reason she doesn’t seem to come to the full realization until her husband death. This leads one to believe that was a common place for women to be unhappy in their marriage and have no conventional means of escape (divorce). However, Chopin doesn’t directly make that point. Many women are in se arch of freedom from their marriage and they believe the only way to be free is to experience the death of their husband. Mrs. Louise Mallard is a repressed married woman that has a heart condition and the reaction to her husband’s presumed death is a sign that she is unhappy. After hearing the tragic news she goes up stairs and looks out an open window and notices â€Å"new spring life†, the delicious breath of rain†, and â€Å" countless sparrows tittering in the eaves.† As she looks out the window among th...

Much Ado About Nothing: Love And Marriage Essay -- essays research pap

Even though love and marriage was a major ideal in Shakespearean England, we can get views from Much Ado about Nothing which oppose this idea. From the two main ‘couples’ in this play we can understand their different views on commitment throughout and because of this we as readers and viewers can learn about each relationship separately and watch the thoughts and ideas change throughout the play. From the scene given we can make many assumptions on the Beatrice and Benedick relationship and how it may have grown throughout the previous acts and scenes. We can tell from this small passage that Beatrice and Benedick have a love/hate relationship which may have come from past relationship let downs†¦ Beatrice: indeed my lord, he lent it me awhile, and I gave him use for it, a double heart for his single one. Marry, once before he won it off me with false dice, therefore your grace may well say I have lost it. The two seem to have the same ideas on marriage even though we learn through the play that this is not true, they both know it is important to get married but have different viewpoints on the matter, they both appear to want their individuality. We can see this idea in the passage provided and many other parts of the play†¦ Beatrice: just, if he send me no husband; for the which blessing I am at him upon my knees every morning and evening. Lord, I could not endure a husband with a beard on his face I had rather lie in the woollen†¦ Benedick: the savage bull may; but i...

Organizational Leadership Essay -- Career Employment Business

Organizational Leadership As a valued and seasoned employee, one is presented with the rare opportunity of determining the type of leadership position that best suits him or her. To some extent this opportunity would be a dream come true. As an experienced employee, one should be familiar with the vision and mission of the organization. One should also be familiar and acclimated to the day to day operations and processes of the company. Identifying ones attributes will better help one define his or her role which will most benefit the success of the organization. When identifying ones strengths and weaknesses, a personal leadership style will assist one in the defining processes for which position will be a perfect fit. Therefore, presented this opportunity sounds awesome, but choosing a career is a tricky business. Where does one start? Is there just one career that would be the perfect match or are there dozens out there waiting to be discovered? â€Å"More often than not, if one chooses a career that doesn’t suit his or her personality one becomes confused, unhappy and burned out† (Lorenz, 2004). No one wants a job he or she dislikes. People want to enjoy going to work because in most cases it takes up a quarter of the day. Settling down with the right career can be as central to your happiness as choosing the right person in a relationship. But with any relationship, there is no right or wrong answers. So instead of choosing a career based on what one thinks he or she should do, self assessments can help one find a career that fits his... ... skills that are needed to lead this new virtual set of team members, I am definitely going to have an immense challenge in my perfect position. References Gratton, L. (2007, June 16). Working Together...When Apart. Retrieved March 13, 2009, from http://online.wsj.com/article/SB118165895540732559-search.html Hefferman, M. (2007, December 19). What You See Is What You Get -- or Is It? Retrieved March 14, 2009, from http://www.fastcompany.com/node/74036/print Lorenz, K. (2004, December 20). Six moves to make more money. Retrieved March 12, 2009, from http://www.cnn.com/2004/US/Careers/08/03/job.burnout/ Shrader, R. W. (2007, Fall). Leadership in a Liquid World. Leadership and Organizational Studies, 49, 96. Retrieved March 14, 2009, from http://sloanreview.mit.edu/smr/issue/2007/fall/17/

Analysis of Mozart’s K. 515 Mvt. 1

Wolfgang Amadeus Mozart Quintet No. 3 in C major, K. 515 Andrew McGuire Dr. Burkart MUSHIS 200 11/19/2012 Wolfgang Amadeus Mozart was a prodigy of his time, and arguable the greatest of all time. This paper will discuss an analysis of his third string quintet in C major, K. 515. Through this piece in Sonata Form we will dissect the exposition, the development, the recapitulation, and the coda; along with an analysis of the quintet we will briefly discuss parts of Mozart’s life, as well as look at the background of the Viola Quintet No. 3 in C Major, K. 515.Wolfgang Amadeus Mozart was born into a musical family after his sister Nannerl, by his father and mother Leopold and Anna Maria. His beginnings would originate in Salzburg, Austria (Eisen). As much is known about the education of little Wolfie we see that his father Leopold is responsible (Jeffery). We also discover that it was not just his musical education that concerned his father but other areas as well, such as arithme tic, reading, writing, and literature (Eisen). Much is not know about the mother of Mozart. She was born into a middle class non-musical family.His father, on the other hand was an accomplished musician and teacher born into life as a choirboy. The nearest compositions to Wolfie’s K. 515 are a second string quintet K. 516 and one of his better known Operas Don Giovanni. Taking a short break from Don Giovanni Mozart would set off to write a pair off string quintets. K. 515 and K. 516. Written the age of 31, the better-known two of the pieces is the g minor quintet (K. 516), and the C major quintet (K. 515) is regarded as the finer of the two. Almost a month would separate the composing of the sister quintets both written for double viola.During the time of the second quartet would be the passing of his father, and some say that there may be a relation between the key of the g minor and the passing (Christiansen). This dual viola is different for the time, even given that Mozar t preferred viola, was seen as odd. The number of his six viola quintets is seen as rare in comparison to the 66 cello quintets written by Boccherini. Even though these quintets maybe rare in amount, many consider them the finest of the string quintet repertoire (Christiansen). The finer of our finest quintets begins in C major. The longest part of the first movement is undoubtedly the exposition.In the first of the one hundred and thirty-one measures of the exposition we see our first theme (see Figure 1). Figure 1 Ninety-four measures later we see our second theme (see Figure 2). Figure 2 Throughout the piece we will see our two themes return continuously in multiple keys. Anytime we see theme one return we see the two lines played between only the first violin and the cello. We see similar segregation for theme two with the first and second violins. The one time we see the violas given a theme to play is in the coda, and will be discussed later, this theme we will see is theme tw o in C major.Listening further we discover that our development turns out to be the shortest part of the piece (Absil). Theme one reappears again in the beginning of the development section, only this time we see the theme appearing in the dominant of the piece (see Figure 3). Figure 3 As the development progresses we also see theme two return as well in its original key, the harmonies we see differently, only briefly because it will be what leads us into the recapitulation (see Figure 4). Figure 4 At the end of our very brief development we arrive at our recapitulation.In the recapitulation we see the return of both of our themes in the tonic key. Throughout the recapitulation we see our theme variations return as well in the tonic key. What is found most notable about the recapitulation is the way it ends. One might assume that it would end on the tonic or dominant, because of its movement to the coda, however we find the end of the recapitulation as a vii °65/V (see Figure 5). Figure 5 We can argue the beginning of the Coda here because of the bar of rest. This theory separates the two sections allowing us to make a clear observation where one ends and the other begins.As with codas we find our final theme in the piece the part proceeding the measure of rest (see Figure 5). Delving deeper into the coda we find theme two reoccurring in the tonic key, this time not segregated by instrument parts. For the first time in the movement we see the two viola parts obviously playing what is the theme (see Figure 6), right before we see the second viola briefly mingle with the cello. Figure [ 6 ] After the brief return of theme two between the cello and violas we end happily on the tonic of the piece. Overall I thoroughly enjoyed this piece.Not only this piece but also this assignment. It is one thing to sit in class and read about and take notes over sonata-allegro form. It is completely another to be expected to dive deeper into a piece and try to figure out its i nner most workings. As a violist I also appreciate the fact that Mozart went against the normality of the cello quintet and wrote a viola quintet. The fact he was willing to go against what was expected of him by society makes me respect him as a composer even more. After a glimpse into the life of Wolfgang Amadeus Mozart, we have discussed his Quintet No. 3 in C major.Discussed its themes and their placement, as well as briefly the variations of these themes. We also saw a brief glimpse into history around the time that the K. 515 and what could be considered its sister K. 516 were written. Works Cited Absil, Frans. â€Å"Musical Analysis: Visiting the Great Composers. † (2005): 8. Absil, the Netherlands. Sept. 2012. Web. 7 Nov. 2012. . Christiansen, Kai. â€Å"String Quintet (viola) No. 3 in C Major, K. 515. † Mozart. Music at Kohl Mansion, 2012. Web. 7 Nov. 2012. . Cliff Eisen, et al. â€Å"Mozart. † Grove Music Online. Oxford University Press. Web. 18 Oct. 2 012. http://www. oxfordmusiconline. com/suscriber/article/grove/music/40258pg3. Gallagher, Sean and Thomas Forrest Kelly. The Century of Bach and Mozart: Perspectives on Histoiography, Composition, Theory, and Performance. Cambridge, Massachusettes: Harvard University Press, 2008. Print. Jeffery, G. â€Å"Mozart, Maria Anna â€Å"Nannerl†Ã¢â‚¬  Mozart, Maria Anna â€Å"Nannerl† N. p. , 1998. Web. 5 Nov. 2012. . Kerst, Friedrich.Mozart: the Man and the Artist Revealed in His Own Words. New York: L. Dover Publications, 1965. Print Mirka, Danut. Metric Manipulations in Haydn and Mozart: Chamber Music for Strings, 1787-1791. Oxford, New York: Oxford University Press, 2009. Print Mozart, W. A. String Quintet No. 3 in C major, K. 515 (I). Alban Berg Quartet. EMI Classics. CD. Mozart, W. A. String Quintet No. 3 in C major, K. 515 (I). Fine Arts Quartet. Musical Concepts. CD Mozart, W. A. Quintet in C Major, K. 515 (I). Wolfgang Amadeus Mozarts Werk, Serie 14: Quartette fuer streichinstrumente. Leipzig: Breitkopf und Haertel, 1877-1910. 1-12. Score.

911

Monday 19th October English course mesh Ross Kemp in Afghanistan and 9/11 docu custodytaries By Sophia Howkins Introduction- In this es narrate I am going to compargon both(prenominal)(prenominal) documentaries to the highest degree(predicate) Ross Kemp in Afghanistan and the 911 crash. twain of these documentaries atomic number 18 re after(prenominal) struggleds-hoursd as they both started beca implement of the corresponding reason. They ar found on the accredited drama on the flesh out and startliness in the forces. The war began on October 7, 2001 the sire of invasion was to realize Osama Bin Laden. British and the U. S military launched in solvent to the 11 attacks in kinsfolk, The 911 happened on Tuesday el dismantleth September 2001 in the morning in the United States.Genre- The archetypal ever infotainment was make in France 1895, at that place was no heavy or colour it was precisely a moving image thither was no editing apply however a capacious take of a exact. The word nonsubjective comes from the word take the stand and hard-nosed come on actor foresightful takes put d avouch deportment in veritable form and formative rise means editing montage ( more than aesthetic on the wholey pleasing). Documentaries in a flash atomic number 18 more formative approach non a realistic approach because they bring in more equipment to edit the objective. scoff documentaries admit caused scepticism in an auditory sense so when it comes to true documentaries the hearing go int believe them.But most documentaries be based on true stories e. g. film wild lifespan. Documentaries be thought to be educational and boring to roughly flock. Ross Kemps docudrama in Afghanistan is a typical objective film because of the atomic number 18a war/political this objective uses both formative approach and realistic because it has whatever editing in. And it is a moderated documental film this infotainment has inform al interviews, the fix of the engageing on a held go cam, synchronous sound account booking and voice over the motion-picture photography. circumstance-The film c wholeed Ross Kemp in Afghanistan is based on the war casualty today, it is over the 9/11 attack on the par each toldel towboats. It has carried on over all these days and it started in 2001 and is stock-still ongoing. The British hosts aim for the attack was to find Laden. The infotainment Ross Kemp in an Afghanistan was Directed by John Conroy and Anuar Arroyo (2nd serial publication) Produced by Matt Bennett. The cardinal series involved Kemp and a niggling embedded film work party future(a) troops competitiveness in Helmand Province, documenting their vox in the ongoing Herrick.In the inaugural series Kemp and his crowd participate in the Vikings initial dressing in Britain for the deployment. They then envision the unit during their six month tour, record both lives at rest and on fighting gu ards in Helmand. The series in the long run covers their return to the UK. In the fol little up series Kemp returns to Afghanistan to assess how the conflict has changed since his starting time visit in 2007. During the filming of the first series, in atomic number 53 and only(a) engagement the crew were pinned win by blaze from the Taliban, with Kemp faithful to dying with bullets passing within inches.In filming the second series, Kemp runs into a possible minefield. The return was p turn ond for the beastliness and realism of the battle footage, and is believed to be the first docudrama film of its kind record in. Audience- The hearing of this objective is junior lads who are deciding to join the regular host or who are already in it. The age audience is 16 to late 30s. Audience has changed over the geezerhood because documentaries use more popular subjects which are attractions a unseasoneder audience. Typical audience over the years are in general citizenry who enjoy watching documentaries.Documentaries all educate or a pursual. viewing audience may have a hobby and like to watch documentaries what interest them or schools use them for educational reasons. Narrative- Ross Kemp in Afghanistan is round Ross Kemp the journalist and narrator doing a documentary on the war in Afghanistan. The documentary is about the soldiers lives in the war, how they drop off their families and wizards, the impact on the soldiers families, the soldiers mental/physical health and how they decease out and train and finally how the Afghanistan civilians impression about living in a war zoneThis documentary is attempt to scan us that troops life is non that true(p). When you watch the documentary it delegates how comfortable we are to have stuff which they gullt have or arent very good e. g. a consume and bed. It in like manner come outs that creation in the army is quite scary because you dont know if youre going to live done the day or if youre not going to overhear your family again. This documentary is creation told by Ross Kemp and his filming crew, the crew films it life and Ross Kemp is the narrator. The British the States and the Taliban are involved in the war.I remember this documentary is unique because it is actually educating us and demo us what it is really like in the war and it should teach people not to take advantage of what they have got. design- at that place are many unlike groups equal in the text the first be the British Army. The British Army in this documentary are being meeted as strong and go outing to fight for our country. Its no all fun and happiness. The soldiers are hit emotionally because of what is happening more or less them. At the setoff they entangle scared, act childish. By being in the army they change the course they act and think.The soldiers cant lodge to put all thither training into action in the war zone. In this documentary the army are fighting for t heir country and the civilians of Afghanistan. When thither on patrol they are grown-up and sea captain only if in the documentary after their time on patrol when Ross Kemp interviewed them they were thinking of leaving the army because now they know it isnt all fun being in the army. Their families have to keep strong for their soldiers. In the documentary there families were described to be unquiet and anxious and loyal they dont leave them they stick by their soldiers.The documentary had a mformer(a) interviewed about her 19 year old son who died on patrol she was strong, devastated and distraught merely she gave Ross Kemp a video of how her son died to go in the documentary. The civilians in Afghanistan were prepared to negotiate with the British Army only when they never gave to often information because they were scared some think will happen to them. The cautions for the civilians are that they are in a middle of a battle zone. The Talibans were described as very tactic al, strong well a d trained. Media Language- photographic camera fractures/ illumine-I am going to talk about a range of camera views. firstly in ray 1 the component part uses both formative approach and realistic approach. It was a birds centre view fissure and it essayed that the land was flea-bitten nothing was to be seen. The main straining more or less was rudimentary it showed how mightily a machine hitman really is. The major powerful feeling is accent because the weapon is closest to the camera. Secondly, picture 3 was a close up of Ross Kemp on the ground lying win. This cinch made you feel claustrophobic and it gives you the Idea of the closeness of the soldiers and how they rely on each some other a lot.Unlike shot 1 this shot does not use deep charge as Ross Kemp is to remain the central focus of this camera shot. We are faced with montage of shots of weapons, action shots, training and frontline Shot 4 was a medium long-shot. Ross Kemp and the sold iers was on the meat cleaver and the door was exhaust the light was glistening through and Ross Kemp walked out. The light was bright and it represented heaven or walking through the tunnel of death or as some people will say walking into the unknown because they dont know what is going to happen at one time they step out align the helicopter.The connotations are to this shot is that its like death because they use the helicopter to take bodies back to England. In shot 7, it the mis en scene is irrelevant uses an extreme close-up of a barbed cable this could have represented danger/keep-out or it was like representing prison for the Afghanistan civilians or peradventure it could be comforting them from danger. The barbed electrify is dark and gloomy exclusively there was a beautiful sunset female genital organ the barbed outfit. The lighting uses juxtaposition between the darkness/ danger of barbed wire and natural beauty of the sunlight.Costumes/Props/Sounds The costume s for this documentary where generally army uniform which is principally good because it camou iris diaphragmes the soldiers because the colours are beiges and browns and creams because of the give up colours. They will be recognised by their blood type which is sewed on to there arm of the uniform mainly because if they get killed they can be recognised thither are lots of weapons in this documentary and whenever theyre in shot there in centre to feel more powerful the weapons become the uniform.The documentary uses synchronous sound (Recording, happening with action) hoi polloi sounds increased to be more powerful and that your there and fear the feelings of the soldiers. The shouting of the soldiers emphasises the chaos. linguistic context- The 9/11 documentary was based on the attack on the couple news written report columns. The 9/11 attack were a series of coordinated suicide attacks by al-Qaeda upon the United States on September 11, 2001. The locations of the attacks were New York metropolis Arlington County, Virginia and near Shanks Ville, Pennsylvania.The types of attack were Aircraft hijacking, Mass mutilate and Suicide attack. It killed 2,976 victims and 19 hijackers and injured 6,000. some(prenominal) structures collapsed within two hours, destroying nearby buildings and electronegative others. The hijackers crashed a third airliner into the Pentagon in Arlington, Virginia, plainly outside Washington, D. C. The fourth matt crashed into a field near Shanks Ville in boorish Pennsylvania, after some of its passengers and flight crew attempted to retake control of the plane, which the hijackers had redirected toward Washington, D. C.There were no survivors from any of the flights. Audience- The audience for this documentary is a little bit variant to the audience from Ross Kemp in Afghanistan, mainly because this documentary is more aimed at firemen or young people deficiencying to join the serve entirely the Ross Kemp is for soldier s. Narrative- 9/11 was originally based on a probe fire fighter called Tony. The documentary was meant to be about a male child becoming a man from his raillery at an early stage into it. The directors for this documentary were two brothers called Jules Naudet and Geodeon Naudet.They didnt mean to record the 9/11 attack. It was an accident. The plane was that humble it caught everyones attention, including the two brothers filming. Planes are never seen just about the area of the duplicate towers. This documentary has real live footage from the horrific terrorist attack. There is emotional footage and interviews with fire fighters who showed their feelings about the attack. A number of them said they would have to analyze to love the job again because of what they all had to go through, digging to find both dead people or survives.Everybody doomed someone or someones close to them because of the attack 9/11 but even though the firemen thoughts were about the safety of their family, they were still determined to turn in other civilians with the dish of the support of the public. just about of the fire fighters had flashbacks of what happened that very day. In the documentary, passim the attack on the repeat towers countersign broadcasts were shown on the news, either from the location or pictures from the viewers. They were edited to fit the documentary to show different sides of the attack.The 9/11 and Ross Kemp in Afghanistan have some similarities. They both used hand held cameras to shoot the action happening around them. In the Ross Kemp documentary they had a helmet camera too. Also they both shoot on the location which the documentaries are about. Both firemen and soldiers time being interviewed questioned their jobs after and season the attack were happening. On the other hand both documentaries have some differences, time the firemen and soldiers were being interviewed they both had different approaches. The 9/11 used formal interviews .They interviewed the inwardness witness after the attack but in Ross Kemps documentary the interviews were informal they enter on the location while the attack was happening. Both of the documentaries have different cultures for utilization the two French narrators expressed their emotions by hugging and kissing each others heads but the American firemen just hugged each other and shacked each others hands. They showed their emotions differently. Representation- There are many different groups represented in the 9/11 documentary. The first being New York firemen from melt down 1.At the beginning they were relaxed and joked around with Tony teasing him. On the job they was represented as serious, brave and professional firemen, while the attacks were happening they seemed shocked, distraught and selfless stalk example one firemen leaned over one of the film-makers to keep him safe and he didnt care about him self. He risked his own life to protect another civilian. alone of t he firemen were very emotional and had open feelings after the attack. Secondly the civilians who were represented as perplexity and shocked, people idnt have a clue what was going on, Felt confounded that they couldnt do nothing, They also felt concern for others around or in the buildings but for them selfs too. third the film-makers, they shared the analogous emotions as the civilians much(prenominal) as scared, helpless and they also frightened but they were brave and for the others they put on a straight face. Also they was reverential because there were two people on fire and they never filmed it but they said to my left there are two people on fire it doesnt feel right to film it.They were professional because they focused on filming and let the film-maker take over. The filming was by two brothers which had different culture to the American. Their emotions are more open than the Americans. They were loyal, love and caring towards each other. The Ross Kemp in Afghanist an and the 9/11 have a hardly a(prenominal) similarities. They both risked there lifes and are seen as heros or angles but also they joke around but remain professional when it counts. The documentaries were both filmed on the location and they both risked their lives to get real footage of the war and attacks.Also the both documentaries have some differences. The firemen are there to save lifes and put out fires but the soldiers are there to kill there targets and protect the civilians of the country there fighting. The firemen are more emotional than the soldiers because if someone died in the army in the war zone they have to leave the dead soldier and choose on with the job but the firemen will carry them or take them to safety. The 9/11 attack was unexpected but the Ross- Kemp was planned. Media Language- television camera Shots-Camera shot one is a pan shot of the people in the street around the pit towers. This shot shows how the attack effected everyone even tourists. Al so it showed all the different life styles from business men to mothers. This shot allows the audience to see the twin towers and how it affected everyone there. This camera shot is a worm eye view shot. This shot shows how large the twin towers and buildings around are but at this point none of the towers had been touched yet. This shot gives us an impression that the twin towers are powerful and the status is looking up at them.Camera shot three, is a close up of the firemen. The reason for this shot is to show the emotions of the firemen, which were shocked and anxious. Also to show their expressions, this shot could represent the firemen asking god for help or they were looking up to heaven, also it could suggest death This camera shot is of the American flag. It is a median low angle shot. This shot of the flag could represent power, primp for the country or by chance its a symbol. When the flag was shown it was meant to show joy and reserve but after the attack the joy died .Tony the probe had to raise the flag everyday and pull it push down every night to show respect, that they still think about there friend who died in a fire nerve-racking to save someone. After the attack on the twin towers Tony had to pull down the flag to show joy that none of his brothers died from the fire station but he didnt want to raise or pull it down again because of all the death that happened that day. Camera shot five is of both twin towers but one has already had the plane crash into it. This shot is a median long shot it also has some mis en scene in it, theres good deal story and befog in this shot.The report is flying around in the air coming down to the ground and settling also the smoke shows confusion because people couldnt see where they were going but there was sidereal day through the top of the chuck forwards and after the attack. There was blue sky one side and the other side dear focus onto one tower at this moment the other tower wasnt touched at this stage. The paper and ashes could show and look like bodies burnet. The paper could also indicate to being confetti but confetti is to celebrate and it represents happiness but in this documentary its about death. Camera shot 6 is a long shot of after the attack.All of the people who were in the attack looked the homogeneous because of the ash, this could suggest that everyone is the same underneath but also it could show that all of the civilians have experienced the same thing. While the ash was falling down to the ground it looked like snow this is ironical because snow is associated with Christmas which Is a time of good will and cheer. Costumes/ Props- Again the 9/11 only had one costume. Which was the firemens uniform, birthing this uniform it gave the firemen sense of pride and a sense of unity and togetherness, because in the 9/11 documentary the firemen talked about being brothers.The uniform also was custodial in case of an emergency. It also gave them power wearin g the uniform, the people of New York and from all over looked up to the fire-fighters for all there hard-work and effort. If the firemen got lost or hurt they wear badges with there fire stations and ladder they work at so people can recognize the fire-fighters, this is similar to the soldiers from Ross- Kemp documentary and the charge they have the regiment and face pack they belong to, on their uniform. Compare to the soldiers the way that their uniform gives them a sense of pride and unity.The civilians clothes reflected that there were people from all over the creative activity from different cultures and countries. This emphasises how it had an impact on everyone not just New-York civilians. The clothes of the civilians mirrored the ending of the twin-towers as clothing got torn and specious further on in the documentary. There were some props. Mainly there was paper floating in the sky from the building also there was business men with brief-cases and woman with shopping bags and pushchairs plus cameras and phones from all of the civilians.